Formation of Nα-benzyloxycarbonyl-l-aminoadipic acid from Nα-benzyloxycarbonyl-l-lysine with mycelia from Aspergillus niger

A method for formation of Nα-benzyloxycarbonyl-l-aminoadipic acid (Nα-Z-l-AAA) was investigated. Nα-Z-l-AAA was formed from Nα-Z-l-lysine via Nα-Z-l-aminoadipate-δ-semialdehyde (Nα-Z-l-AASA) by the reaction with mycelia from Aspergillus niger according to Scheme 1. In this reaction, oxidation of Nα-Z-l-lysine to Nα-Z-l-AASA was catalyzed by amine oxidase. The reaction at pH 7.0 and 30 °C was optimum for Nα-Z-l-AAA formation, and approximately 50% of the decomposed Nα-Z-l-lysine was converted into Nα-Z-l-AAA.