Author/Authors :
Horii، نويسنده , , Ken and Adachi، نويسنده , , Takashi and Matsuda، نويسنده , , Tetsuya S. Tanaka، نويسنده , , Tsutomu and Sahara، نويسنده , , Hiroshi and Shibasaki، نويسنده , , Seiji and Ogino، نويسنده , , Chiaki and Hata، نويسنده , , Yoji and Ueda، نويسنده , , Mitsuyoshi and Kondo، نويسنده , , Akihiko، نويسنده ,
Abstract :
β-Glucosidase (BGL1) from Aspergillus oryzae was efficiently produced in recombinant A. oryzae using sodM promoter-mediated expression system. The yield of BGL1 was 960 mg/l in liquid culture, which is 20-fold higher than the yield of BGL1 produced using the yeast Saccharomyces cerevisiae. Recombinant BGL1 converted isoflavone glycosides into isoflavone aglycones more efficiently than β-glucosidase from almond. In addition, BGL1 produced isoflavone aglycones even in the presence of the insoluble form of isoflavone glycosides.
Keywords :
?-glucosidase , aglycone , Aspergillus oryzae , Superoxide dismutase (sodM) promoter , Isoflavone
