Title of article :
Expression of rat diamine oxidase in Escherichia coli
Author/Authors :
Rosini، نويسنده , , Elena and Nossa، نويسنده , , Serena and Valentino، نويسنده , , Mattia and D’Arrigo، نويسنده , , Paola and Marinesco، نويسنده , , Stéphane and Pollegioni، نويسنده , , Loredano، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2012
Abstract :
In this work, a reliable protocol was designed to rapidly express and purify a rat diamine oxidase in Escherichia coli as a useful alternative to enzyme isolated from animal sources. The cDNA encoding for rat diamine oxidase was overexpressed in an Origami2(DE3) E. coli strain and, by employing a rapid purification protocol in which the hexahistidine tag was added at the C-terminal end of the enzyme, the recombinant oxidase could be purified in a single step on a Ni-NTA column at >95% purity. The enzyme was active but was largely produced in an immature quinone form: Cu2+ ions stimulated further activation/maturation. This expression and purification procedure offers an easy and rapid means of producing recombinant rat diamine oxidase from an animal-free source and represents a useful tool to boost biotechnological application of this enzyme.
Keywords :
amine oxidase , Animal-free enzyme , TPQ maturation , Mammalian TPQ enzyme , Amines assay
Journal title :
Journal of Molecular Catalysis B Enzymatic
Journal title :
Journal of Molecular Catalysis B Enzymatic