Kinetics of adsorption of lipase onto different mesoporous materials: Evaluation of Avrami model and leaching studies

Mesoporous silica materials were synthesized by hydrothermal route and covalently modified with glutaraldehyde. The adsorption (chemical adsorption and physical adsorption) of enzyme Candida rugosa lipase onto pure and functionalized mesoporous silica materials having different pore diameter were studied. Kinetics of adsorption was evaluated by Avrami model and specific kinetic parameters n and k were determined experimentally. Leaching studies revealed that chemical adsorption via covalent binding was less prone to leaching. Maximum loading capacity of each mesoporous material was analyzed and the result showed that MCF-25G has highest adsorption capacity. It was also observed that MCF-25G has highest stability towards enzyme binding.