Author/Authors :
Uji-i، نويسنده , , Hiroshi and Melnikov، نويسنده , , Sergey M. and Deres، نويسنده , , Ania and Bergamini، نويسنده , , Giacomo and De Schryver، نويسنده , , Frans and Herrmann، نويسنده , , Andreas and Müllen، نويسنده , , Klaus and Enderlein، نويسنده , , Jِrg and Hofkens، نويسنده , , Johan، نويسنده ,
Abstract :
Defocused wide-field fluorescence microscopy was used to follow the 3D molecular rotational diffusion of a fluorescent probe molecule in a polymer thin film. The technique allows for visualizing the molecular reorientation both in-plane and out-of-plane. The local environmental change driven by heterogeneous dynamics of the polymer can be probed on a scale of 1 μm as demonstrated by parallel imaging of several molecules. A multi-component rotational diffusion decay is observed which might reflect both different relaxation regimes of the polymer as well as rapid changes of the local environment.
