Title of article :
pH-change protective PB-b-PEO polymersomes
Author/Authors :
Mueller، نويسنده , , Waltraut and Koynov، نويسنده , , Kaloian and Pierrat، نويسنده , , Sebastien and Thiermann، نويسنده , , Raphael and Fischer، نويسنده , , Karl and Maskos، نويسنده , , Michael، نويسنده ,
Issue Information :
دوهفته نامه با شماره پیاپی سال 2011
Pages :
5
From page :
1263
To page :
1267
Abstract :
Poly(butadiene)-b-poly(ethylene oxide) vesicles were successfully loaded with hydrophilic dye Phloxine B. Dye addition during vesicle formation leads to Phloxine B encapsulated inside the water filled vesicle core as well as to freely diffusing dye molecules. The removal of uncapsulated substrate involves time consuming methods like dialysis or harsher methods like ultra filtration or selective precipitation, posing the risk of irreversible sample manipulation. Here used Phloxine B as pH sensitive fluorescence indicator allows the characterization of hydrophilic loading without separation procedure by adjusting the pH value. Additionally membrane blocking efficiency can be studied by time dependent fluorescence measurements. Cryogenic TEM studies showed that the self-assembled structure remained unchanged when the hydrophilic dye was incorporated within the vesicles. Fluorescence microscopy imaging proved the encapsulation of the hydrophilic dye inside the core volume. The combination of fluorescence correlation spectroscopy (FCS) and dynamic light scattering (DLS) measurements as ensemble methods confirmed those results additionally.
Keywords :
diblock copolymers , Self assembly , Vesicle loading
Journal title :
Polymer
Serial Year :
2011
Journal title :
Polymer
Record number :
1737135
Link To Document :
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