One-pot synthesis of amphiphilic nanogels from vinylated SPIONs/HEMA/PEG via a combination of click chemistry and surfactant-free emulsion photopolymerization: Unveiling of the protein-nanoparticle interactions

In this study, freshly prepared Fe3O4 nanoparticles (MNP1) were coated with 3-aminopropyltriethoxysilane (APTES) to produce core–shell Fe3O4@SiO2 nanoparticles (MNP2), amine terminated nanoparticles was converted into the triazide in the presence of as-prepared triflic azide (MNP3). Propargyl acrylate (PgA) was synthesized from propargyl alcohol and acryloyl chloride and their structures were characterized by FT-IR and 1H NMR spectroscopy. MNP3 were modified by PgA via click reaction to produce fully decorated triazole product (MNP4). Photopolymerization of MNP4 in the presence of hydroxyethyl methacrylate (HEMA) and acrylated methyl ether poly (ethylene glycol) (ACMPEG) were carried out by emulsion method without any surfactant (MNP5). The in-vitro release behavior of quercetin from MNP5s was investigated at two pHs (7.4 and 5.8). The effect of fetal bovine serum (FBS) on MNPs and its ability to cover magnetite nanoparticles was investigated.