Title of article :
Modulation of collagen II fiber formation in 3-D porous scaffold environments
Author/Authors :
Tan، نويسنده , , Guak-Kim and Dinnes، نويسنده , , Donna Lee M. and Cooper-White، نويسنده , , Justin J.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2011
Abstract :
Collagen II, a major extracellular matrix component in cartilaginous tissues, undergoes fibrillogenesis under physiological conditions. The present study explored collagen II fiber formation in solution and in two- (coverslip) and three-dimensional (scaffold) environments under different incubation conditions. These conditions include variations in adsorption buffers, the presence of 1-ethyl-3-(3-dimenthylaminopropyl) carbodiimide/N-hydroxysuccinimide crosslinker and the nature of the material surfaces. We extend our observations of collagen II fiber formation in two dimensions to develop an approach for the formation of a fibrillar collagen II network throughout surface-modified polylactide-co-glycolide porous scaffolds. Morphologically, the collagen II network is similar to that present in native articular cartilage. Biological validation of the resultant optimized functional scaffold, using rat bone marrow-derived mesenchymal stem cells, shows appreciable cell infiltration throughout the scaffold with enhanced cell spreading at 24 h post-seeding. This economic and versatile approach is thus believed to have significant potential in cartilage tissue engineering applications.
Keywords :
Surface modification , EDAC/NHS crosslinking , Collagen II , 2-D and 3-D environments , fibrillogenesis
Journal title :
Acta Biomaterialia
Journal title :
Acta Biomaterialia