ANALYSIS OF ANTIBIOTIC RESISTANT GENES IN BACTERIA

Antibiotic resistance is a serious and growing phenomenon in contemporary medicine and has emerged as one of the pre-eminent public health concern. In this work we have isolated, identified the bacteria from Burn wound samples. Later the antibiotic genes were transferred to E.Coli to study the antibiotic mechanism. Grams staining, Endospore staining test, capsulated staining test, Motility test were carried out for the morphology of cell. Catalase, ONPG, Lysine decarboxylase, Ornithine, Urease, Phenyl alanine deamination, Nitrate reduction, H2S production, Citrate utilization, Voges proskaeurs, Methyl red, Indole and Malonate were suited for biochemical studies. Plasmid DNA was isolated and Plasmid curing was performed inorder to identify the position of Antibiotic genes. The microorganism identified was staphylococcus and the antibiotic resistant genes were present in the Plasmid. The genes were isolated and transffered to E.coli and E.coli which was sensitive to antibiotics showed antibiotic resistance.