Title of article :
Connexin36 (Cx36) expression and protein detection in the mouse carotid body and myenteric plexus
Author/Authors :
Frinchi، نويسنده , , Monica and Di Liberto، نويسنده , , Valentina and Turimella، نويسنده , , Sada and D’Antoni، نويسنده , , Francesca and Theis، نويسنده , , Martin and Belluardo، نويسنده , , Natale and Mud?، نويسنده , , Giuseppa، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2013
Pages :
5
From page :
252
To page :
256
Abstract :
Although connexin36 (Cx36) has been studied in several tissues, it is notable that no data are available on Cx36 expression in the carotid body and the intestine. The present study was undertaken to evaluate using immunohistochemistry, PCR and Western blotting procedures, whether Cx36 was expressed in the mouse carotid body and in the intestine at ileum and colon level. In the carotid body, Cx36 was detected as diffuse punctate immunostaining and as protein by Western blotting and mRNA by RT-PCR. Cx36 punctate immunostaining was also evident in the intestine with localization restricted to the myenteric plexus of both the ileum and the colon, and this detection was also confirmed by Western blotting and RT-PCR. All the data obtained were validated using Cx36 knockout mice. Taken together the present data on localization of Cx36 gap-junctions in two tissues of neural crest-derived neuroendocrine organs may provide an anatomical basis for future functional investigations.
Keywords :
Cx36 , GAP JUNCTIONS , mouse , connexin , Myenteric plexus , Carotid Body
Journal title :
Acta Histochemica
Serial Year :
2013
Journal title :
Acta Histochemica
Record number :
1760161
Link To Document :
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