Characterization of electrochemically synthesized poly(2-fluoroaniline) film and its application to glucose biosensor

Poly(2-fluoroaniline) has been electrochemically deposited on ITO coated glass plates in the form of thin films using 4 M perchloric acid as electrolyte. These polymeric films have been spectroscopically characterized by FT-IR and UV-visible spectrophotometric methods. Room temperature electrical conductivity of the polymeric films was measured and found to be lower than that of polyaniline films. The SEM studies show the blunt needle like surface morphology of the polymeric film. e oxidase (GOX) has been immobilized on to these electrochemically deposited conducting poly(2-fluoroaniline) films by physical adsorption method. The redox characterization of poly(2-fluoroaniline) and poly(2-fluoroaniline)/GOX films has been carried out by cyclic voltammetry technique. Amperometric detection of β-glucose was carried out by using the above enzyme immobilized polymeric film as a working electrode and Ag/AgCl as a reference electrode at a bias voltage of 0.2 V over a physiological range. The electrode carrying GOX was found to be stable up to 32 days.