Fluorescence quenching kinetic model for a bound and partitioned quencher in micelles

A kinetic model for quenching of a fluorescent probe in micelles is analysed. The probe is totally incorporated into the micelles. The quencher distributes between water and the micellar pseudophase in two different ways; i.e. by binding and by partition. The model is analytically solved under the assumption that quenching of the probe is much faster than the quencher exchange either between the two phases or between partition and binding sites within the same micelle. Equations for the time-resolved and steady-state fluorescence intensities are derived. The model is applied to the quenching of a carboxylated zinc phthalocyanine fluorescence by diethyl-aniline in CTAC micelles.