Author/Authors :
Watanabe، نويسنده , , Takeshi and Iketaki، نويسنده , , Yoshinori and Omatsu، نويسنده , , Takashige and Yamamoto، نويسنده , , Kimihisa and Ishiuchi، نويسنده , , Shun-ichi and Sakai، نويسنده , , Makoto and Fujii، نويسنده , , Masaaki، نويسنده ,
Abstract :
We have demonstrated a realistic super-resolution scanning fluorescence microscope using conventional nanosecond lasers. This super-resolution microscope is based on the combination of two-color fluorescence dip spectroscopy and shape modulation to a doughnut beam. Only by introducing a doughnut erase beam, the resolution of the laser fluorescence microscope breaks the diffraction limit by two times without using any mechanical probe.