On the methods of determination of homogeneous nucleation rates of protein crystals

A recently published analysis of an experimental method to directly determine the homogeneous rates of nucleation of protein crystals or other solid phases of proteins inferred that the method underestimates the homogeneous nucleation rates by several orders of magnitude. Here, we consider the presented arguments and find that the analysis contains certain inconsistencies. We conclude that the homogeneous nucleation rate data produced using the direct determination technique are, most likely, correct.