Morphological and macrostructural studies of dog cranial bone demineralized with different acids

Strong and weak acids were chosen for the bone decalcification process. Demineralization of dog cranial bone was carried out using 0.6 M concentration of either of hydrochloric, lactic or citric acid. Consequent morphologic changes were correlated with the developed chemistry, porosity and structure through XRD and FT-IR of the matrices. The dielectric permittivity, loss angle, relaxation dielectric loss as well as a.c. electrical conductivity as functions of frequency and temperature were measured. Zeta potential was evaluated at physiologic pH and temperature and correlated with the developed structures. sults prove lower dielectric properties of demineralized matrices compared to control and exhibited high dissipation of electric energy with more than one relaxation mechanism. This variation proves that the spectral behavior depended on the nature of the bone matrix which resulted from the phase compositions of bone and its crystallite size. The behavior of the obtained results is attributed to the differences of the demineralizing acids and their consequent actions on the matrices, i.e., the differences in the materials.