Development of Water Quality Test Kit Based on Substrate Utilization and Toxicity Resistance in River Microbial Communities

Methods for measuring toxicity or respiratory activity of microbial cultures can be used as tools for assessing the presence of chemicals and their impact on the streams. The proposed toxicity test is based on the respirometric characteristics of the bacteria according to the principals of Biolog’s microplate system. As the microorganism are utilizing the carbon source (peptone), the reduction of the tetrazolium dye as the redox indicator is taking place, leading to a developing change in the well’s color The tests provided us with information in regard to the toxicity range of the chemicals with activated sludge and Alcaligenes.