Title of article
Control of neural stem cell differentiation on honeycomb films
Author/Authors
Tsuruma، نويسنده , , Akinori and Tanaka، نويسنده , , Masaru and Yamamoto، نويسنده , , Sadaaki and Shimomura، نويسنده , , Masatsugu، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2008
Pages
5
From page
536
To page
540
Abstract
Control of neural stem cell (NSC) differentiation is ongoing interest in neural tissue engineering. Formation of neural networks on various patterned substrates was reported in previous studies. In this study, we cultured NSCs derived from the cerebral cortex of embryonic day-14 mice on honeycomb (HC) films with highly regular pores prepared by casting a polymer solution of water-immiscible solvent under high humidity. The differentiation of NSCs was analyzed by immunostaining for Nestin and MAP2. The differentiation of NSC was controlled for the first time by manipulating the pore size on HC films. The highest suppression of NSC differentiation was observed on HC film with 3 μm pore specifically.
Keywords
Cell differentiation , Neural regeneration , Neural stem cell , Micro and nano-pattern , Neuron
Journal title
Colloids and Surfaces A Physicochemical and Engineering Aspects
Serial Year
2008
Journal title
Colloids and Surfaces A Physicochemical and Engineering Aspects
Record number
1795980
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