Author/Authors :
Miyashiro، نويسنده , , Isao and Kaname، نويسنده , , Tadashi and Nakayama، نويسنده , , Takahiro and Nakamori، نويسنده , , Shoji and Yagyu، نويسنده , , Toshio and Monden، نويسنده , , Takushi and Kikkawa، نويسنده , , Nobuteru and Nishisho، نويسنده , , Isamu and Muramatsu، نويسنده , , Takashi and Monden، نويسنده , , Morito and Akiyama، نويسنده , , Tetsu، نويسنده ,
Abstract :
Midkine (MK) is a growth differentiation factor originally found as the product of a retinoic acid-responsive gene. The expression of MK was examined in 35 surgically resected specimens of primary colorectal cancer using the reverse transeription-polymerase chain reaction (RT-PCR). All of the cancerous tissues expressed MK. In 5/25 cancerous tissues a truncated form of MK, which was recently found in various human tumor cell lines, was detected in addition to the full-size MK. In contrast, the truncated form of MK could not be detected in non-cancerous tissues, whereas the wild-type form was detected in 8/10 non-cancerous tissues. These results suggest that the expression of the truncated form of MK may be associated with tumorigenesis.
Keywords :
Growth factor , Midkine , Truncated form , Alternative splicing , Reverse transcription-polymerase chain reaction , Colorectal Cancer
