Title of article
Rapid cytocidal and cytostatic chemosensitivity test by measuring total amount of mRNA
Author/Authors
Miura، نويسنده , , Yasuhiko and deFries، نويسنده , , Rici and Shimada، نويسنده , , Hiroshi and Mitsuhashi، نويسنده , , Masato، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 1997
Pages
6
From page
139
To page
144
Abstract
Chemosensitivity of vinblastin, cisplatin, and mitomycin C were assessed in four different human cancer cell lines (U937, HL-60, CaR-1, and HepG2) by MTT (3-(4,5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide) assay and the measurement of total cytosolic poly(A)+ mRNA. Results of 12 h mRNA assay with 10× peak plasma concentration (PPC) were significantly correlated with that of standard 3 days MTT assay with 1× PPC. Furthermore, mRNA assay was changed more significantly than MTT assay under cytostatic condition. Because of its minimum culture requirement (12 h) and broad spectrum (cytocidal and cytostatic), mRNA assay will become a useful tool for chemosensitivity test.
Keywords
Chemosensitivity , cancers , MRNA , Yoyo-1 , MTT
Journal title
Cancer Letters
Serial Year
1997
Journal title
Cancer Letters
Record number
1798507
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