Author/Authors :
Korkut، نويسنده , , Ca?atay and Yalç?ntepe، نويسنده , , Leman and Kiremit-Korkut، نويسنده , , Ne?e and Uzun-Alt?n?z، نويسنده , , Semire and ??sever، نويسنده , , Saliha and Gümü?el، نويسنده , , Füsun and Tiryaki، نويسنده , , Demir and Bermek، نويسنده , , Engin، نويسنده ,
Abstract :
NAD+ glycohydrolase activities in serum samples from cancer patients were two- to three-fold higher than the activities in samples from healthy controls. SDS-PAGE analysis of serum samples followed by Western blotting revealed the presence of two proteins of ~45 and ~21 kDa that were immunoreactive with human CD38-specific monoclonal antibodies T16, HIT2 and OKT10. These proteins appeared to be more abundant in serum from cancer patients. NAD+ glycohydrolase activity in serum could be enriched by immunoaffinity chromatography by using T16-Sepharose 4B. The results suggest that the relative abundance of proteins immunologically related to CD38 may account for the elevated levels of glycohydrolase activities in serum of tumour patients.
Keywords :
Tumour patients , Serum NAD+ glycohydrolase , ADP-ribosylation , CD38-specific monoclonal antibody , CD38
