Title of article
Human in vitro cell lines verification by minisatellite DNA restriction fragment length polymorphism
Author/Authors
Ma?czak، نويسنده , , Maria and Zieli?ska-Dawidziak، نويسنده , , Magdalena and Prussak، نويسنده , , Magdalena and Dubis، نويسنده , , Joanna and Ko??، نويسنده , , Anita and Du?، نويسنده , , Danuta and Wojdat، نويسنده , , El?bieta and Ku?nierczyk، نويسنده , , Piotr، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 1998
Pages
8
From page
113
To page
120
Abstract
Four families of human in vitro cell lines were tested for minisatellite restriction fragment length polymorphism (RFLP) using multilocus probes MZ1.3 and/or 33.15 after digestion of DNA with restriction enzymes HinfI or HaeIII. These results confirmed that (i) the RFLP pattern is relatively stable in established cell lines and, therefore, could be used as a specific marker of a cell line identity, (ii) the use of MZ1.3 and 33.15 probes permits the identification of hybridomas and (iii) one of the cell lines tested, a lymphoblastoid cell line HAJ, may possess a hot spot of mutation.
Keywords
Minisatellite DNA , Human cell lines , Mutation , restriction fragment length polymorphism
Journal title
Cancer Letters
Serial Year
1998
Journal title
Cancer Letters
Record number
1799519
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