Author/Authors :
Ma?czak، نويسنده , , Maria and Zieli?ska-Dawidziak، نويسنده , , Magdalena and Prussak، نويسنده , , Magdalena and Dubis، نويسنده , , Joanna and Ko??، نويسنده , , Anita and Du?، نويسنده , , Danuta and Wojdat، نويسنده , , El?bieta and Ku?nierczyk، نويسنده , , Piotr، نويسنده ,
Abstract :
Four families of human in vitro cell lines were tested for minisatellite restriction fragment length polymorphism (RFLP) using multilocus probes MZ1.3 and/or 33.15 after digestion of DNA with restriction enzymes HinfI or HaeIII. These results confirmed that (i) the RFLP pattern is relatively stable in established cell lines and, therefore, could be used as a specific marker of a cell line identity, (ii) the use of MZ1.3 and 33.15 probes permits the identification of hybridomas and (iii) one of the cell lines tested, a lymphoblastoid cell line HAJ, may possess a hot spot of mutation.
Keywords :
Minisatellite DNA , Human cell lines , Mutation , restriction fragment length polymorphism
