An immunohistochemical study of the effects of surgical induction of anterior disc displacement in the rabbit craniomandibular joint on type I and type II collagens

The right craniomandibular joint (CMJ) was exposed surgically and all the discal attachments severed except for the posterior one. The disc was then repositioned anteriorly and sutured to the zygomatic arch. The left joint served as a sham-operated control; 10 other joints were used as non-operated controls. Deeply anaesthetized rabbits were perfused with 2% buffered formalin 2 weeks (10 rabbits) or 6 weeks (10 rabbits) after the induction of the anterior disc displacement (ADD). The articular disc, bilaminar zone, mandibular condyle and articular eminence were excised. The condyles and the articular eminences were demineralized in EDTA. All tissues were then sectioned at 10 μm in a cryostat. Sections were incubated with polyclonal antibodies directed against type I or type II collagens. Following incubation in the appropriate fluorescein isothiocyanate-labelled secondary antibodies, these specimens were studied under the fluorescence microscope. At 2 weeks there was a reduction in type II collagen immunostaining; some areas of the experimental condylar cartilage showed a switch from type II to type I collagen. However, at 6 weeks there was an increase in type II collagen immunostaining and a decrease in type I compared to the 2-week group. It is concluded that surgical induction of ADD in the rabbit CMJ leads to alteration in the condylar cartilage collagen phenotype similar to that reported for osteoarthritic cartilage of other synovial joints.