Type VI collagen periodic fibrils in the synovium of the mouse temporomandibular joint

Immunohistochemical staining with anti-type VI collagen antibody was strongly positive in the intimal layer and moderately positive in the subsynovium. After treatment with 20 mM ATP, numerous structures with a periodicity of 100 ± 10 nm (type VI collagen fibrils) appeared around the synovial cells. As the periodic dark bands were stained by ruthenium red, proteoglycan(s) or glycosaminoglycan(s) were probably associated with the type VI collagen fibrils. When the tissue was digested with testicular hyaluronidase before ATP treatment, the periodic fibrils were not found, and only a filamentous network of 100-nm interval was seen around the cells. Thus, type VI collagen is abundant in the synovium of the mouse mandibular joint and is associated with proteoglycans or glycosaminoglycans, which might have a role in its formation.