Title of article :
Inhibition of DNA synthesis by downregulation of cyclin A but not Skp 2 overexpression in human hepatocellular carcinoma cells
Author/Authors :
Chao، نويسنده , , Yee and Shih، نويسنده , , Yung-Luen and Chen، نويسنده , , Hsin-Ju and Lee، نويسنده , , Shou-Dong and Huang، نويسنده , , Tze-Sing and Kuo، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1999
Pages :
6
From page :
1
To page :
6
Abstract :
Cyclin A is an S and G2/M phase regulatory protein and associates with Skp 2 in many transformed cells. Our previous results showed that 12 (39%) and 17 (55%) out of 31 hepatocellular carcinoma (HCC) patients exhibited higher protein expression levels of cyclin A and Skp 2, respectively, in their tumorous compared to non-tumorous tissues. In the present study, we used Western blot analysis to show that 3 out of 6 HCC cell lines, HA59T, HA22T and HCC36, exhibited overexpression of cyclin A, among which the HCC36 cell line also expressed a higher Skp 2 protein level. Moreover, we used the antisense oligonucleotide phosphorothioates to down regulate the overexpression of cyclin A and Skp 2 proteins to determine whether or not these two proteins are involved in the mitogenesis of HCC36 cells. After treatment with antisense oligonucleotide phosphorothioates, the gene product of cyclin A or Skp 2 was suppressed dose-dependently as revealed by Western blot analyses. By [3H]thymidine incorporation assay, we found that downregulation of cyclin A but not Skp 2 overexpression could inhibit the DNA synthesis ability of HCC36 cells, suggesting that abnormal Skp 2 expression is not directly correlated with the HCC proliferation.
Keywords :
Antisense oligonucleotide phosphorothioate (S-oligo) , Hepatocelluar carcinoma (HCC) , DNA synthesis , Cyclin A , Skp 2
Journal title :
Cancer Letters
Serial Year :
1999
Journal title :
Cancer Letters
Record number :
1800238
Link To Document :
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