Methylation of exon 2 of p16 is associated with late stage oesophageal cancer

Methylation of the p16 gene was studied in 16 oesophageal tumours. Five (31%) of the tumours were found to be methylated in exon 1 and eight (50%) were methylated in exon 2. The loss of p16 protein correlated with methylation of exon 1 (P=0.005). However, methylation of exon 2, but not exon 1, was found to be associated with late stage tumours (P=0.01). We conclude that the methylation of exon 2 of p16 may have effects on the progression of oesophageal tumours that are independent of the expression of the p16 protein.