Author/Authors :
Noguchi، نويسنده , , Yoshikazu M. Saito، نويسنده , , Aya and Miyagi، نويسنده , , Yohei and Yamanaka، نويسنده , , Shoji and Marat، نويسنده , , Doulet and Doi، نويسنده , , Chiharu and Yoshikawa، نويسنده , , Takaki and Tsuburaya، نويسنده , , Akira and Ito، نويسنده , , Takaaki and Satoh، نويسنده , , Shinobu، نويسنده ,
Abstract :
We attempted to suppress glucose transporter 1 (GLUT1) expression by transfecting MKN45 cells with cDNA for antisense GLUT1. Glucose transport was significantly decreased in cells with antisense GLUT1 compared with wild-type cells or cells with vector alone. Suppression of GLUT1 mRNA resulted in a decreased number of cells in the S phase. This was accompanied by overexpression of p21 protein. Tumorigenicity in the nude mice injected with antisense GLUT1 expressing cells was significantly slower than in those with wild-type MKN45 cells. These results suggest that antisense GLUT1 mRNA inhibits tumor growth through a G1 arrest and that expression of antisense GLUT1 mRNA via gene therapy can be used as a tool in the treatment of cancer.
