Localization of neuronal-constitutive nitric oxide synthase and secretory regulation by nitric oxide in the rat submandibular and sublingual glands

The distribution of neuronal-constitutive nitric oxide synthase (ncNOs)-positive nerve fibres was compared immunohistochemically, and the effect of NOs inhibitor and NO scavenger on the secretory response was compared functionally, in the two glands. Numerous ncNOs-positive fibres were distributed around acini in the submandibular gland but scarcely any around acini in the sublingual gland. Within the submandibular ganglion (parasympathetic), the nerve-cell bodies were strongly positive. Within the superior cervical ganglion (sympathetic), the nerve-cell bodies were negative, although some positive nerve fibres were observed. The secretory responses to the electrical stimulation of the chorda were significantly reduced by the NOs inhibitor, NG-nitro-l-arginine methyl ester (L-NAME, 10−9–10−3 M) in a dose-dependent manner. The NO scavenger, 2-(4-carboxyphenyl)-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide (carboxy-PTIO) also reduced the chorda-evoked secretion (10−9–10−6 M). The submandibular secretions evoked by stimulation of the superior cervical ganglion were not affected by L-NAME or carboxy-PTIO. In the sublingual gland, neither L-NAME nor carboxy-PTIO affected chorda-evoked salivary secretion. The histochemical and functional results both suggest that NO plays an excitatory role in the regulation of parasympathetic nerve-induced salivary secretion in the rat submandibular gland, but not in the sublingual gland.