Author/Authors :
Yano، نويسنده , , Tomohiro and Yajima، نويسنده , , Shoko and Virgona، نويسنده , , Nantiga and Yano، نويسنده , , Yoshihisa and Otani، نويسنده , , Shuzo and Kumagai، نويسنده , , Hiromi and Sakurai، نويسنده , , Hidetoshi and Kishimoto، نويسنده , , Mikako and Ichikawa، نويسنده , , Tomio، نويسنده ,
Abstract :
The present study was undertaken to estimate the effect of 6-methylthiohexyl isothiocyanate (6MHITC) isolated from Wasabia japonica (wasabi) pretreatment on 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone(NNK)-induced lung tumorigenesis in mice. Pretreatment with 6MHITC for 4 consecutive days at a daily dose of 5 μmol significantly inhibited NNK-induced O6-methylguanine formation in lungs at 4 h after the injection. In conjugation with this inhibitory effect, 6MHITC suppressed the increase in proliferating nuclear cell antigen level as well as ornithine decarboxylase activity at a promotion stage of NNK-induced lung tumorigenesis. Finally, this treatment of 6MHITC suppressed the NNK-induced lung tumorigenesis in mice. These results suggest that 6MHITC inhibits the development of lung tumors in mice treated with NNK, due to the suppression of initiation stage.
Keywords :
ornithine decarboxylase , tumorigenesis , proliferating cell nuclear antigen , 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone , Lung , 6-Methylhexylisothiocyanate
