Title of article :
Protein tyrosine kinase inhibitor, genistein, enhances apoptosis and cell cycle arrest in K562 cells treated with γ-irradiation
Author/Authors :
Papazisis، نويسنده , , Konstantinos T. and Zambouli، نويسنده , , Dimitra and Kimoundri، نويسنده , , Ourania T. and Papadakis، نويسنده , , Emmanuel S. and Vala، نويسنده , , Vasiliki and Geromichalos، نويسنده , , George D. and Voyatzi، نويسنده , , Soultana and Markala، نويسنده , , Dimitra and Destouni، نويسنده , , Evangelia and Boutis، نويسنده , , Lazaros and Kortsaris، نويسنده , , Alexander H.، نويسنده ,
Abstract :
Genistein, is a natural isoflavone compound with a potent activity against protein tyrosine kinases. The leukemic cell line, K562, is a bcr/abl (Philadelphia chromosome) positive cell line that is resistant to DNA-damaging agents, including γ-irradiation. Treatment with genistein increased apoptosis and promoted G2-phase arrest in the non-apoptotic population of the γ-irradiated K562 cells. Irradiated cells that survived 72 h after the irradiation had a normal distribution in cell cycle, whilst genistein treatment kept cells arrested in the G2-phase, decreased the S-phase fraction and suppressed DNA-synthesis. Taken together, our results show that genistein augments apoptotic cell death after γ-irradiation in K562 cells and this result cannot be attributed to abrogation of the G2/M checkpoint.
Keywords :
K562 , ?-Irradiation , cell cycle , apoptosis , Genistein
