DNA histochemical analysis in oral squamous cell carcinoma

SummaryBackground years have witnessed an increasing emphasis on the role of nuclear DNA and its application in experimental pathological diagnosis to predict prognosis and management of certain neoplasm. ablish objective criteria for the degree of differentiation and histochemical quantitative of nuclear DNA of oral squamous cell carcinoma (OSCC) using microspectrophotometric analysis. al and methods udy was conducted on histologic materials from patient with OSCC. Two histological grading systems were used; Broderʹs and invasive front grading system were recorded. Microspectrophotometry was applied on Feulgen-stained sections to determine the quality of tumour nuclear DNA content in two different histological grading systems of OSCC. s r DNA content increased significantly with decreasing tissue differentiation as well with increasing tumour size. sion ading system and DNA content provides more objective and accurate criteria which relate the morphologic finding to biologic activity and growth patterns of oral cancer as compared to histologic differentiation alone.