Author/Authors :
Hanafusa، نويسنده , , Tadashi and Yumoto، نويسنده , , Yasuhiro and Nouso، نويسنده , , Kazuhiro and Nakatsukasa، نويسنده , , Harushige and Onishi، نويسنده , , Toru and Fujikawa، نويسنده , , Tatsuya and Taniyama، نويسنده , , Mayumi and Nakamura، نويسنده , , Shinichiro and Uemura، نويسنده , , Masayuki and Takuma، نويسنده , , Yoshitaka and Yumoto، نويسنده , , Eiichiro and Higashi، نويسنده , , Toshihiro and Tsuji، نويسنده , , Takao، نويسنده ,
Abstract :
Insulin-like growth factor binding protein-3 (IGFBP-3) is postulated to be a mediator of growth suppression signals. Reduced expression of the IGFBP-3 was observed in nine out of 12 human hepatocellular carcinomas (HCC) (75%). Promoter hypermethylation of the IGFBP-3 was detected in four out of 12 HCCs (33%) although mutations were not identified. The expression of IGFBP-3 was restored by the demethylating agent 5-aza-2′-deoxycytidine in HCC cell line with promoter hypermethylation (HepG2). As IGFBP-3 functions like a tumor suppressor gene, it may be used as a therapeutic target for HCC.
Keywords :
hepatocellular carcinoma , Hypermethylation , Insulin-like growth factor binding protein-3 , cDNA microarray , Mannose 6-phosphate/insulin-like growth factor 2 receptor
