Title of article :
Consistent downregulation of human lactoferrin gene, in the common eliminated region 1 on 3p21.3, following tumor growth in severe combined immunodeficient (SCID) mice
Author/Authors :
Yang، نويسنده , , Ying and Li، نويسنده , , Jingfeng and Szeles، نويسنده , , Anna and Imreh، نويسنده , , Marta P and Kost-Alimova، نويسنده , , Maria H.B. Kiss، نويسنده , , Hajnalka and Kholodnyuk، نويسنده , , Irina N. Fedorova، نويسنده , , Ludmilla and Darai، نويسنده , , Eva and Klein، نويسنده , , George and Imreh، نويسنده , , Stefan، نويسنده ,
Abstract :
Lactoferrin (LF) is one of 19 active genes in the common eliminated region 1 at 3p21.3 identified by us. LF was transfected into mouse fibrosarcoma A9. Fourteen severe combined immunodeficient (SCID) derived tumors from two PI based artificial chromosome (PAC)-transfectants containing the entire LF gene and two LF-cDNA transfectants were analyzed by real time polymerase chain reaction at the DNA and RNA level. Following SCID tumor passage, LF expression was decreased or eclipsed, in all tumors although DNA levels did not change considerably. Promoter methylation and/or rearrangement of the insertion site may be responsible for human LF downregulation in mouse fibrosarcoma derived tumors.
Keywords :
3p21.3 , Common eliminated region 1 (CER1) , Human lactoferrin gene , Elimination test , Real time-polymerase chain reaction
