Title of article :
Cyclooxygenase-2 expression in squamous dysplasia and squamous cell carcinoma of the esophagus
Author/Authors :
Yu، نويسنده , , Hongping and Xu، نويسنده , , Shun-Qing and Liu، نويسنده , , Li and Shi، نويسنده , , Luyuan and Cai، نويسنده , , Xiao-Kun and Lu، نويسنده , , Wen-Hong and Lu، نويسنده , , Bin and Su، نويسنده , , Yanhua and Li، نويسنده , , Yuan-Yuan، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2003
Pages :
9
From page :
193
To page :
201
Abstract :
Cyclooxygenase-2 (cox-2) overexpression has been observed in several types of human cancers and has been implicated in carcinogenesis. To elucidate the role of cox-2 in esophageal carcinogenesis, we evaluated the expression of cox-2 in normal squamous epithelium squamous epithelial dysplasia (n=47), and squamous cell carcinoma of the esophagus (n=86) by immunohistochemistry, reverse transcription-PCR assay, and western blotting. A significant overexpression of cox-2 was observed in esophageal squamous dysplasia and squamous cell carcinoma compared with normal squamous epithelium. The immunoreactive score of cox-2 expression, an index determined by intensity and positivity of cox-2 staining, was 0.71±0.46 (mean±SD) in normal squamous esophagus, 2.19±1.79 in squamous epithelial dysplasia, and 2.67±1.77 in squamous cell carcinoma. The results of immunohistochemistry were confirmed by a reverse transcription-PCR assay and western blotting analysis. Cox-2 expression level was correlated with proliferation activity assessed by proliferating cell nuclear antigen (PCNA) index and MIB-1 index in dysplastic lesion (r=0.55, P<0.01 with PCNA and r=0.72, P<0.01 with MIB-1) and carcinoma (r=0.56, P<0.01 with PCNA and r=0.72, P<0.01 with MIB-1). Elevated cox-2 expression was associated with high p53 expression (p<0.001) but not with clinicopathological features including age, sex, tumor size, histological grade, lymph node metastasis, and TNM stage. The results indicated that cox-2 may be involved in an early stage of squamous carcinogenesis of the esophagus, and that cox-2 overexpression was related to cell proliferation in esophageal squamous dysplasia and squamous cell carcinoma.
Keywords :
Squamous epithelial dysplasia , SQUAMOUS CELL CARCINOMA , Cyclooxygenase-2 , Esophagus , immunohistochemistry
Journal title :
Cancer Letters
Serial Year :
2003
Journal title :
Cancer Letters
Record number :
1805437
Link To Document :
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