Title of article :
Efficacy of enzymatic toothpastes for immobilisation of protective enzymes in the in situ pellicle
Author/Authors :
Hannig، نويسنده , , C. and Spitzmüller، نويسنده , , B. Matijasevic-Lux، نويسنده , , H.C. and Altenburger، نويسنده , , M. and Al-Ahmad، نويسنده , , A. and Hannig، نويسنده , , M.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2010
Pages :
7
From page :
463
To page :
469
Abstract :
Aim ent enzyme-containing toothpastes are available on the market. The aim of the present in situ study was to investigate their efficacy for immobilisation of protective enzymes in the pellicle layer. s le formation took place in situ on bovine enamel slabs fixed to individual upper jaw splints carried by 6 subjects. After pellicle formation for 1 min, brushing was performed for 3 min with the commercially available toothpastes Enzycal, biotène and BioXtra, respectively. Before as well as 0, 20 and 40 min after brushing, samples were removed from the splints and tested for lysozyme, peroxidase and glucoseoxidase activity. The assays for the respective enzyme activities were based on fluorogenic substrates. Separate experiments were conducted for the different enzymes and toothpastes. s ng with the toothpastes caused an extensive increase of glucoseoxidase activity in the pellicle, but it was of low tenacity whereas peroxidase activity was enhanced considerably. However, targeted accumulation of lysozyme in the pellicle was not very pronounced. Brushing without toothpaste had no effect on enzyme activities in the acquired pellicle. sion ed immobilisation of enzymes in the in situ pellicle can be achieved with toothpastes.
Keywords :
Tooth paste , Lysozyme , Peroxidase , Enzyme , Pellicle , Glucoseoxidase , immobilisation
Journal title :
Archives of Oral Biology
Serial Year :
2010
Journal title :
Archives of Oral Biology
Record number :
1805723
Link To Document :
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