Title of article
Hypermethylation of the PTEN gene in ovarian cancer cell lines
Author/Authors
Ron Schondorf، نويسنده , , Thomas and Ebert، نويسنده , , Matthias P. and Hoffmann، نويسنده , , Juliane and Becker، نويسنده , , Martina and Moser، نويسنده , , Natasha and Pur، نويسنده , , ?aziye and G?hring، نويسنده , , Uwe-Jochen and Weisshaar، نويسنده , , Maria-Paz، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2004
Pages
6
From page
215
To page
220
Abstract
This study was performed to investigate the hypermethylation status of the PTEN gene in ovarian cancer. To this end, we incubated eight ovarian cancer cell lines with the demethylating agent 5-aza-2′ deoxycytidine in three different concentrations for 5 days. Subsequently, the PTEN expression was quantified by both real time RT-PCR and quantitative western analyses. PTEN mRNA varied considerably in response to demethylation whereas PTEN protein concentrations remained constant in all cell lines except OAW42 cells (12.5%). The data suggest that PTEN is highly regulated at translational level. However, methylation of the PTEN gene plays a subordinate role in ovarian cancer.
Keywords
Ovarian cancer , Methylation , PTEN
Journal title
Cancer Letters
Serial Year
2004
Journal title
Cancer Letters
Record number
1806324
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