Author/Authors :
Toshimitsu، نويسنده , , Hiroaki and Hashimoto، نويسنده , , Kiichiro and Tangoku، نويسنده , , Akira and Iizuka، نويسنده , , Norio and Yamamoto، نويسنده , , Kohtaro and Kawauchi، نويسنده , , Shigeto and Oga، نويسنده , , Atsunori and Furuya، نويسنده , , Tomoko and Oka، نويسنده , , Masaaki and Sasaki، نويسنده , , Kohsuke، نويسنده ,
Abstract :
To clarify the molecular basis of acquired cisplatin (CDDP) resistance in esophageal squamous cell carcinoma (ESCC), we used cDNA microarray technology. A CDDP-resistant cell line (YES-2/CDDP), which shows a 7.5-fold increase in resistance to CDDP and a 3-fold decrease in CDDP accumulation compared with the parental YES-2 ESCC cell line, was generated from YES-2 by exposure to increased concentrations of CDDP. By cDNA microarray analysis, we identified 44 genes with significantly different expression levels between YES-2/CDDP and YES-2 cells. Interestingly, 15 of these 44 genes encoded ribosome-related proteins, almost all of which were underexpressed in YES-2/CDDP cells. Our present data suggest that many ribosome-related genes may be involved in the acquired resistance to CDDP in ESCC and that such information may allow us to better understand the mechanism of CDDP resistance.
Keywords :
Acquired CDDP-resistant cell line , cDNA microarray , ribosomal protein , comparative genomic hybridization
