Title of article :
Infection of human gingival fibroblasts with Aggregatibacter actinomycetemcomitans: An in vitro study
Author/Authors :
Arirachakaran، نويسنده , , Pratanporn and Apinhasmit، نويسنده , , Wandee and Paungmalit، نويسنده , , Proudchompoo and Jeramethakul، نويسنده , , Pawika and Rerkyen، نويسنده , , Pimprapa and Mahanonda، نويسنده , , Rangsini، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2012
Abstract :
Objective
atibacter actinomycetemcomitans is known to be a major cause of localized aggressive periodontitis. Previous research has suggested that A. actinomycetemcomitans can damage many types of host cells. There is evidence for the ability of this organism to invade endothelial and epithelial cells, but information pertaining to its potential for invading gingival fibroblasts is very limited. Internalization of bacteria is not only responsible for damaging host tissue but also a means to evade the host immune response. It was hypothesized that A. actinomycetemcomitans can invade and reside in human gingival fibroblasts (HGF).
s
y cultures of HGF were infected with A. actinomycetemcomitans at a ratio of 1:100. Bacterial internalization was determined by an antibiotic protection assay. Bacterial-fibroblast interaction was examined using phase-contrast, scanning and transmission electron microscopy.
s
demonstrated that A. actinomycetemcomitans was internalized into HGF at an efficiency of 0.084%. Transmission electron microscopic study showed the presence of A. actinomycetemcomitans in the cytoplasm of HGF without the surrounding membrane. Scanning electron micrographs revealed the sloughing of HGF surfaces on which A. actinomycetemcomitans adhered. Rounded cells, attachment loss and damaged cells were also observed.
sions
concluded that the attachment and invasion of A. actinomycetemcomitans into human gingival fibroblasts play a role in periodontal tissue damage and may also be a means of immune evasion.
Keywords :
Aggregatibacter actinomycetemcomitans , Infection , Human gingival fibroblasts , Electron microscopy
Journal title :
Archives of Oral Biology
Journal title :
Archives of Oral Biology