Author/Authors :
Li، نويسنده , , Ge and Kim، نويسنده , , Dong-Hee and Kim، نويسنده , , Tae-Dong and Park، نويسنده , , Byoung-Jeon and Park، نويسنده , , Hae-Duck and Park، نويسنده , , Jong-Il and Na، نويسنده , , Min-Kyun and Kim، نويسنده , , Hwan-Chul and Hong، نويسنده , , Nam-Doo and Lim، نويسنده , , Kyu and Hwang، نويسنده , , Byung-Doo and Yoon، نويسنده , , Wan-Hee، نويسنده ,
Abstract :
The cytotoxic mechanism of protein-bound polysaccharide isolated from Phellinus linteus (PL, Mesima®) has been investigated. PL inhibited the proliferation and colony formation of SW480 human colon cancer cells. Flow cytometry analysis showed that PL increased the populations of both apoptotic sub-G1 and G2/M phase. The result obtained from TUNEL assay corroborated apoptosis which was shown in flow cytometry. Western blot analysis suggested that PL-induced apoptosis and growth inhibition were associated with decrease in Bcl-2, increase of the release of cytochrome c, and reduced expression of cyclin B1. These results suggest that PL has a direct antitumor effect through apoptosis and cell cycle blockade in certain cancer cells.
Keywords :
Pl , apoptosis , Antitumor effect , G2/M phase arrest
