Author/Authors :
Nakamura، نويسنده , , Hiroyuki and Mukai، نويسنده , , Mutsuko and Komatsu، نويسنده , , Keiko and Tanaka-Okamoto، نويسنده , , Miki and Itoh، نويسنده , , Yu and Ishizaki، نويسنده , , Hiroyoshi and Tatsuta، نويسنده , , Masaharu and Inoue، نويسنده , , Masahiro and Miyoshi، نويسنده , , Jun، نويسنده ,
Abstract :
We have previously shown that transforming growth factor-β1 (TGF-β1) markedly stimulates the invasive capacity of rat ascites hepatoma AH130 W1 cells in vitro and in vivo. A differential hybridization procedure was used to isolate genes that were specifically up-regulated in TGF-β1 treated W1 cells. Among ten independent cDNA clones, we focused on LMO7 and a variant isoform, LMO7S, that was generated by alternative splicing. LMO7 had PDZ and LIM domains, while LMO7S had only PDZ domain. TGF-β1 up-regulated expression levels of LMO7 and LMO7S. LMO7 expression was up-regulated in the highly metastatic clone MM1.
Keywords :
Differential hybridization , Invasion , LMO7 , TGF-?1
