Author/Authors :
Miyata، نويسنده , , Shiro and Uchinokura، نويسنده , , Shunro and Fukushima، نويسنده , , Tsuyoshi and Hamasuna، نويسنده , , Ryouichi and Itoh، نويسنده , , Hiroshi and Akiyama، نويسنده , , Yutaka and Nakano، نويسنده , , Shinichi and Wakisaka، نويسنده , , Shinichiro and Kataoka، نويسنده , , Hiroaki، نويسنده ,
Abstract :
Hepatocyte growth factor activator inhibitor type-1 (HAI-1) is an integral-membrane proteinase inhibitor. In this study, we examined the effects of HAI-1 on human glioblastoma cells. Two glioblastoma cell lines (YKG-1, U251) were stably transfected with expression plasmid harboring mature membrane-form or truncated secreted-form HAI-1. Culture characteristics were not altered by the expression of HAI-1, whereas in vitro invasiveness of U251 was suppressed. On the other hand, the expression of membrane-form HAI-1 resulted in significantly enhanced tumorigenicity of both cell lines in vivo. In contrast, secreted-form HAI-1 did not promote the tumorigenicity. These results suggest that HAI-1 may play complex roles in progression of glioblastoma cells, and membrane-form HAI-1 may mediate an undefined important signaling in the cells.
Keywords :
Glioblastoma , hepatocyte growth factor (HGF) , protease inhibitor , HGF activator , HAI-1
