Author/Authors :
Galy، نويسنده , , Oivier and Petit، نويسنده , , Marie-Anne and Benjelloun، نويسنده , , Soumaya and Chevallier، نويسنده , , Philippe and Chevallier، نويسنده , , Michelle and Srivatanakul، نويسنده , , Petcharin and Karalak، نويسنده , , Anant and Carreira، نويسنده , , Christine and Lyandrat، نويسنده , , Nicole and Essaid، نويسنده , , Abdellah and Trepo، نويسنده , , Christian and Hainaut، نويسنده , , Pierre and Chemin، نويسنده , , Isabelle، نويسنده ,
Abstract :
Detection and localization of Hepatitis C Virus (HCV) in liver tissue is useful for diagnostic purposes as well as to elucidate the mechanisms by which the virus participates in hepatocarcinogenesis. However, so far, a sensitive method for HCV detection at the cellular level is lacking. We describe here the application of a novel antibody, D4.12.9, developed against serum-derived HCV RNA-positive particles, for the detection of E2 proteins by immunohistochemistry in fixed, archived specimens including liver biopsies of HCV-infected patients and surgical specimens of hepatocellular carcinoma. We demonstrate that D4.12.9 is a powerful tool for sensitive and specific detection of HCV, independently of viral genotype. This approach has applications to diagnosis as well as exploratory pathological studies.
Keywords :
Hepatitis C , hepatocellular carcinoma , Monoclonal anti-E2 , immunohistochemistry , Paraffin-embedded tissue , Envelope protein
