Author/Authors :
Scintu، نويسنده , , Marina and Vitale، نويسنده , , Rita and Prencipe، نويسنده , , Maria and Pia Gallo، نويسنده , , Antonietta and Bonghi، نويسنده , , Loriana and Valori، نويسنده , , Vanna Maria and Maiello، نويسنده , , Evaristo and Rinaldi، نويسنده , , Monica and Signori، نويسنده , , Emanuela and Rabitti، نويسنده , , Carla and Carella، نويسنده , , Massimo and Dallapiccola، نويسنده , , Bruno and Altomare، نويسنده , , Vittorio and Fazio، نويسنده , , Vito M. and Parrella، نويسنده , , Paola، نويسنده ,
Abstract :
In a series of invasive ductal breast carcinoma, we investigated the status of chromosomal and intrachromosomal instability by fluorescence in situ hybridisation and determined the level of mRNA expression for two genes involved in the mitotic spindle checkpoint pathway, BUB1B and MAD2L1. All breast cancers demonstrated higher chromosomal instability rates in tumor samples (average: 56.86%, range: 36.24–76.78%) than in controls (average: 11.54%, range: 9.91–14.84%) (P < 0.0001). As well as intrachromosomal instability rates were elevated in tumor (average: 18.45% range: 8.34–35.8%) as compared with controls (average: 4.18% range: 3.47–4.81%) (P < 0.0001). An increase in BUB1B and MAD2L1 transcripts was demonstrated in the majority of the tumor tested. BUB1B mRNA levels but not MAD2L1 levels correlated with intrachromosomal instability (r = 0.722, P = 0.018).
Keywords :
breast cancer , Genomic instability , BUB1B , Gene expression , MAD2L1
