Title of article :
Radiation-induced apoptosis of tumor cells is facilitated by inhibition of the interaction between Survivin and Smac/DIABLO
Author/Authors :
Ogura، نويسنده , , Aki and Watanabe، نويسنده , , Yasuko and Iizuka، نويسنده , , Daisuke and Yasui، نويسنده , , Hironobu and Amitani، نويسنده , , Makoto and Kobayashi، نويسنده , , Saori and Kuwabara، نويسنده , , Mikinori and Inanami، نويسنده , , Osamu، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 2008
Pages :
11
From page :
71
To page :
81
Abstract :
To investigate the mechanism of radioresistance of solid tumor cells, we created two expression vectors encoding Survivin mutants, T34A and D53A. When T34A and D53A were overexpressed in NIH3T3, A549 and HeLa cells, radiation-induced apoptosis was significantly enhanced. Furthermore, we examined the binding capability of Survivin with Smac/DIABLO in the cells that overexpressed these mutants. Coimmunoprecipitation analysis revealed that mutant form of Survivin, D53A and T34A could bind to Smac/DIABLO, but with much less affinity compared to the authentic form. These results suggest that radiation-induced apoptosis of tumor cells is increased by inhibition of the interaction between Survivin and Smac/DIABLO through overexpression of T34A and D53A.
Keywords :
radiation , apoptosis , Adenoviral vector , Survivin , Dominant-negative mutant
Journal title :
Cancer Letters
Serial Year :
2008
Journal title :
Cancer Letters
Record number :
1811241
Link To Document :
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