Title of article :
A novel amplification target, ARHGAP5, promotes cell spreading and migration by negatively regulating RhoA in Huh-7 hepatocellular carcinoma cells
Author/Authors :
Gen، نويسنده , , Yasuyuki and Yasui، نويسنده , , Kohichiroh and Zen، نويسنده , , Keika and Nakajima، نويسنده , , Tomoaki and Tsuji، نويسنده , , Kazuhiro and Endo، نويسنده , , Mio and Mitsuyoshi، نويسنده , , Hironori and Minami، نويسنده , , Masahito and Itoh، نويسنده , , Yoshito and Tanaka، نويسنده , , Shinji and Taniwaki، نويسنده , , Masafumi and Arii، نويسنده , , Shigeki and Okanoue، نويسنده , , Takeshi and Yoshikawa، نويسنده , , Toshikazu، نويسنده ,
Abstract :
RhoA, a member of the Rho family of small GTPases, directs the organization of the actin cytoskeleton and is involved in regulating cell shape and movement. Its activity is negatively regulated by p190-B RhoGAP (GTPase-activating protein). We investigated DNA copy number aberrations in human hepatocellular carcinoma and esophageal squamous cell carcinoma cell lines using a high-density oligonucleotide microarray and found a novel amplification at chromosomal region 14q12. We identified ARHGAP5 (the gene encoding p190-B RhoGAP) as a probable target for the amplification at 14q12, and our results showed that p190-B RhoGAP promotes cells spreading and migration by negatively regulating RhoA activity in Huh-7 hepatocellular carcinoma cells.
Keywords :
ARHGAP5 , RhoA , p190-B RhoGAP , hepatocellular carcinoma , Amplification
