Author/Authors :
Liang، نويسنده , , Shufang and Gong، نويسنده , , Fengming and Zhao، نويسنده , , Xinyu and Wang، نويسنده , , Xianhuo and Shen، نويسنده , , Guobo and Xu، نويسنده , , Yuhuan and Yang، نويسنده , , Hanshuo and Ruan، نويسنده , , Xuzhi and Wei، نويسنده , , Yuquan، نويسنده ,
Abstract :
In order to study the characterization of a new tumor-relative FAM92A1-289 protein, we first constructed plasmid FAM92A1-pQE30 for fusion expression in Escherichia coli. The recombinant protein FAM92A1-289 was affinity-purified by Ni2+-charged resin and separated by HPLC chromatography with high purity, and it was further identified by electrospray ionization-mass spectrometry. Furthermore, the expression and cell localization of FAM92A1-289 by immunohistochemistry using our self-prepared polyclonal antibody showed it was expressed in cytoplasm of renal carcinoma. FAM92A1-289 mRNA was expressed in 2 of 10 kidney tissues and in 6 of 12 primary renal tumors. FAM92A1-289 can promote cell growth in vitro and in vivo by colony formation and mouse xenograft assay. Our present data indicated FAM92A1-289 is a new tumor-related gene with oncogenic potentials to probably play roles in renal carcinogenesis.
Keywords :
His-tagged affinity purification , Prokaryotic expression , polyclonal antibody , FAM92A1-289 protein , renal cell carcinoma
