Author/Authors :
Paris، نويسنده , , Pamela L. and Kobayashi، نويسنده , , Yasuko and Zhao، نويسنده , , Qiang and Zeng، نويسنده , , Wei and Sridharan، نويسنده , , Shivaranjani and Fan، نويسنده , , Tina and Adler، نويسنده , , Howard L. and Yera، نويسنده , , Emmanuel R. and Zarrabi، نويسنده , , M.H. and Zucker، نويسنده , , Stanley and Simko، نويسنده , , Jeffry and Chen، نويسنده , , Wen-Tien and Rosenberg، نويسنده , , Jonathan، نويسنده ,
Abstract :
Circulating tumor cells (CTCs) hold promise for studying advanced prostate cancer. A functional collagen adhesion matrix (CAM) assay was used to enrich CTCs from prostate cancer patients’ blood. CAM ingestion and epithelial immuno-staining identified CTCs, which were genotyped using oligonucleotide array comparative genomic hybridization. The highest CTC counts were observed in men with metastatic castration resistant prostate cancer (CRPC) compared to castration sensitive prostate cancer. Copy number profiles for CRPC CTCs were similar to paired solid tumor DNA, and distinct from corresponding DNA from the residual CAM-depleted blood. CAM CTC enrichment may allow cellular and genetic analyses in prostate cancer.
Keywords :
prostate cancer , metastasis , Blood micrometastases , Circulating tumor cells
