Title of article
A VALIDATED STABILITY-INDICATING HPLC ASSAY METHOD FOR ACYCLOVIR IN BULK DRUG
Author/Authors
Chaudhari، Vilas نويسنده Department of Chemistry, Yogeshwari Mahavidyalaya, Ambejogai. (MS), India. , , Ubale، Milind نويسنده Department of Chemistry, Vasantrao Naik Mahavidyalaya, Aurangabad-431003 (MS), India. ,
Issue Information
فصلنامه با شماره پیاپی سال 2012
Pages
8
From page
5
To page
12
Abstract
An isocratic reversed phase stability-indicating high-performance liquid chromatographic (HPLC) assay method was developed and validated for quantitative determination of Acyclovir in bulk drugs. An isocratic, reversed phase HPLC method was developed to separate the drug from the degradation products, using an Water Nova Pack C18 (250 x 4.6) mm, 5µ column and the mobile phase containing 1.0gm Sodium dihydrogen phosphate and 1.0 gm 1-octaneSulfonic acid salt in 1000ml water filter and mixed. Prepare a homogenous mixture of buffer, methanol and acetonitrile (50:20:30,v/v/v). The detection was carried out at wavelength 264 nm. The developed method was validated with respect to linearity, accuracy (recovery), precision, system suitability, selectivity, robustness prove the stability indicating ability of the method.
Journal title
International Journal of Analytical, Pharmaceutical and Biomedical Sciences
Serial Year
2012
Journal title
International Journal of Analytical, Pharmaceutical and Biomedical Sciences
Record number
1814720
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