Title of article :
Dietary lipid and iron modify normal colonic mucosa without affecting phospholipase A2 activity
Author/Authors :
Donald F. Kuratko، نويسنده , , Connye N. and Pence، نويسنده , , Barbara C.، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1995
Pages :
7
From page :
181
To page :
187
Abstract :
Phospholipase A2 (PLA2) functions as the rate-limiting step in arachidonic acid metabolism and in the removal of damaged or peroxidized membrane lipids. It is elevated in some human tumors and may be involved with mechanisms of tumor promotion. In vitro systems have shown PLA2 activity to be altered by variations in fatty acid and antioxidant components. This study encompassed two objectives. First, PLA2 activity in colon tumors produced using azoxymethane (AOM) in Fischer-344 rats was examined. Secondly, this study tested the effect of iron supplementation as a potential pro-oxidant in diets of varied fatty acid composition on PLA2 activity. Diets included 35 or 140 mg/kg of iron in AIN-76A-based diets high in corn oil, menhaden oil, or beef tallow. Results for the first objective showed PLA2 activity to be significantly higher in colon tumors than in normal mucosa with the increase due primarily to an increase in activity within a paniculate subcellular fraction. In the second objective, fatty acid composition of colon mucosa was altered by both dietary fat and iron. Animals fed beef tallow had the highest level of oleic acid and corn oil-fed animals had the highest level of linoleic acid. Animals fed menhaden oil had the lowest level of arachidonic acid and highest level of α-linolenic, eicosapentaenoic, docosapentaenoic, and docosahexaenoic acids. Iron supplementation in diets high in corn oil resulted in decreased membrane composition of palmitoleic and δ-linolenic acid. In spite of these changes in membrane composition, there were no changes in PLA2 activity. These results show that PLA2 activity is increased in AOM-induced tumors but that diet alone does not influence PLA2 activity in this model.
Keywords :
dietary fat , Dietary iron , Colon , Colon tumor , Phospholipase A2
Journal title :
Cancer Letters
Serial Year :
1995
Journal title :
Cancer Letters
Record number :
1814902
Link To Document :
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