OXIDATIVE STRESS AND ANTIOXIDANTS IN CHRONIC RENAL FAILURE WITH AND WITHOUT DIALYSIS

The present study was designed to demonstrate oxidative stress in chronic renal failure (CRF) patients with the progression of the disease. Plasma advanced oxidative protein products (AOPP), a novel marker of oxidative damage to proteins and % hemolysis an indirect marker of lipid peroxidation were estimated in CRF patients with and without dialysis.Antioxidant status was evaluated by determining GSH, total thiols, albumin and total antioxidant activity in plasma.All the parameters were estimated by spectrophotometric methods. Data was compared with that of age and sex matched controls.AOPP increased significantly in plasma of CRF patients along with %hemolysis compared to controls.% hemolysis significantly increased in hemodialysis patients compared to nondialysed CRF patients. Exhaustion of antioxidants in plasma was evident with significant decrease in albumin, GSH and total thiols in CRF patients as compared to healthy controls. Globulin levels increased significantly in these patients. Plasma albumin decreased significantly with the progression of renal failure. The oxidant: antioxidant imbalance may contribute to the development of pathogenic changes in chronic renal failure.