Ion implantation of negative ions for cell growth manipulation and nervous system repair

We use a negative-ion implantation technique for surface modification for novel biological purpose. Negative-ion implantation has an advantage of “charge-up free” property of implanted material surfaces when negative ions are implanted into an insulator such as polymers for biomaterial use. We performed the modification of polymer surfaces so as to enhance the growth of neurons when cultured on the modified surfaces by negative ions such as carbon, silver, copper etc. Regions of growth enhancement can be formed on the surface, thus providing a tool for selective and enhanced neuron cell growth. This approach to neuronal growth stimulation could offer a technique for constructing an artificial neuron network for future biomaterial science. For another approach, we used it as a tool for bringing about the reconnection and growth of severed nerve bundles in living animals. Experiments include the modification of plastic tube by negative-ion implantation and the evaluation of the nerve regeneration in the modified nerve grafting plastic tube in living animals (rat).